Composite

Part:BBa_K1085023:Design

Designed by: Claudio Tiecher   Group: iGEM13_Groningen   (2013-09-16)

RBS Start-codon EstA Strep-tag SpiderSilkSubunitE1 SpiderSilkSubunitE2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 650
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 262
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence was codon-optimized for Bacillus subtilis.

Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.


Source

The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008) and are from the MaSp2 of the Argiope aurantia.

The DNA sequence was synthesized by Integrated DNA Technologies (IDT).


References

Brooks, A. E., Stricker, S. M., Joshi, S. B., Kamerzell, T. J., Middaugh, C. R., & Lewis, R. V. (2008). Properties of synthetic spider silk fibers based on Argiope aurantia MaSp2. Biomacromolecules, 9(6), 1506–10